Figure 1: Experimental Plate containing Ampicillin, BL21 E. Coli, and plasmid pGEM-gbr22. Incubated at 37 degrees Celsius overnight. Plate is overgrown and shows no independent colonies.
Figure 2: Control Plate containing only Ampicillin and BL21 E. Coli. Incubated at 37 degrees Celsius overnight. Aside from condensation, no growth is present.
Figure 3: Fun Plate containing bacteria swabbed from computer keys in lab. Incubated at 37 degrees Celsius overnight. No Ampicillin present.
Figure 4: Large culture of BL21 E. Coli with plasmid pGEM-gbr22 in a 125mL Erlenmeyer flask. Purple color indicates that bacteria were successfully transformed.
Figure 5: Wet pellet of BL21 E. Coli in a 50mL conical tube after centrifugation and decanting. Pellet weight was 0.35 grams.
Figure 6: Elutions 1 and 2 in 15mL conical tubes after column filtration.
Figure 7: Wet Gel suspended in Nanopure water after staining with 6x loading dye. From left to right: Elution 1, Elution 2, wash, flow through, soluble fraction, cell lysate, ladder.
Figure 8: Dry gel with cellophane. From left to right: ladder, cell lysate, soluble fraction, flow through, wash, Elution 1, Elution 2.
Figure 1: Experimental Plate containing Ampicillin, BL21 E. Coli, and plasmid pGEM-gbr22. Incubated at 37 degrees Celsius overnight. Plate is overgrown and shows no independent colonies.
Figure 2: Control Plate containing only Ampicillin and BL21 E. Coli. Incubated at 37 degrees Celsius overnight. Aside from condensation, no growth is present.
Figure 3: Fun Plate containing bacteria swabbed from computer keys in lab. Incubated at 37 degrees Celsius overnight. No Ampicillin present.
Figure 4: Large culture of BL21 E. Coli with plasmid pGEM-gbr22 in a 125mL Erlenmeyer flask. Purple color indicates that bacteria were successfully transformed.
Figure 5: Wet pellet of BL21 E. Coli in a 50mL conical tube after centrifugation and decanting. Pellet weight was 0.35 grams.
Figure 6: Elutions 1 and 2 in 15mL conical tubes after column filtration.
Figure 7: Wet Gel suspended in Nanopure water after staining with 6x loading dye. From left to right: Elution 1, Elution 2, wash, flow through, soluble fraction, cell lysate, ladder.
Figure 8: Dry gel with cellophane. From left to right: ladder, cell lysate, soluble fraction, flow through, wash, Elution 1, Elution 2.